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miR-382-5p Blocks the Differentiation of NB4 Cells Through Targeting PTEN
Liu Dongdong1,2, Liu Beizhong1,2, Yuan Zhen2, Yao Juanjuan1, Zhong Pengqiang1, Liu Junmei1, Yao Shifei1, Zhao Yi1, Liu Lu2, Chen Min1, Li Lianwen1, Zhong Liang2*
1Central Laboratory of Yong-Chuan Hospital, Chongqing Medical University, Chongqing 402160, China;
2Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Department of Laboratory Medicine,
Chongqing Medical University, Chongqing 400016, China
Abstract: In order to investigate the regulatory effects and mechanism of PTEN (phosphatase and tensin homologue) and miR-382-5p on ATRA (all-trans retinoic acid)-induced differentiation of acute promyelocytic leukemia cell line NB4. Enforced expression of PTEN in APL cells (NB4) and no-APL cells (HL-60， THP-1) was achieved through a lentivirus vector. The miR-382-5p mimic， inhibitor and negative controls were infected into NB4 cells using Lipofectamine 2000. ATRA， a typical regent was to induced granulocytic differentiation. The protein levels of CD11b， PTEN were detected by Western blot. The mRNA expression of miRNA-382-5p and PTEN was measured by quantitative Real-time polymerase chain reaction (qRT-PCR). Data showed that overexpression of PTEN promoted ATRA-induced differentiation in APL cell line NB4 compared to no-APL line HL-60 and THP-1. Enforced expression of miR-382-5p attenuated expression of PTEN and cell differentiation marker CD11b. Conversely， down-regulation of miR-382-5p， increased the expression of PTEN and CD11b. It was concluded that miR-382-5p modulated ATRA-induced differentiation of APL cells by regulating its potential target PTEN.
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