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The Expression of a Novel R-loop in Mouse Mitochondrial DNA during Developmental Phase
Ji-Xia Li, Sheng-Li Li, Xia Meng, Yi-Nong Liu, Ju Wang*
Department of Laboratory Animal Science, Capital Medical University, Beijing 100069, China
Abstract: A novel RNA species that is coded by and complementary to heavy-strand at the mtDNA D-loop region combines with DNA double strand， forming a stable DNA-RNA hybrid (R-loop) spanning the whole H-strand of D-loop region in mouse mtDNA. In this study， we detected the temporal-spatial expression pattern of R-loop in mouse mitochondrion by RT-PCR and Northern blot. An age-dependent increase of R-loop and the differences of R-loop among tissues were found. The differential spatial and temporal expression pattern of R-loop indicates that the biological function of R-loop might be a regulator to control mitochondrial genome expression.
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