Progress of CRISPR/Cas9 System for Gene Editing

Abstract: Accurate editing of plant genomes using genetic editing techniques is a very important method for plant molecular breeding to improve crop traits and yields. The CRISPR/Cas9 system is originated from the acquired immune defense system of Pseudomonas aeruginosa. Because of its high efficiency， easy operation and low cost， the system stands out from many gene editing technologies and is widely favored by researchers. Using CRISPR/Cas9 technology to edit crop genomes for accurately introducing or improving target traits provides a new approach to crop genetics and breeding. It has been reported that CRISPR/Cas9 technology has been widely used in Arabidopsis， rice， potato， corn and other crops. This paper briefly describes the structure， mechanism and differences of zinc finger nuclease (ZFN)， transcriptional activation-like effector nuclease (TALEN)， CRISPR/ Cas9 system， focusing on the progress of the CRISPR/Cas9 system， its improved CRISPR/Cpf1 technology and its advantages as well as limitations compared to other nucleases.

CSTR: 32200.14.cjcb.2018.12.0016