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Growth Inhibition Effect of Onconase on B16 Melanoma Cells in Vivo and in Vitro
Ru-Ling Shen1,2, Rui-Lin Sun3, Qing-Cheng Wang2, Ling Ou1, Jian Fei2*
1State Key Laboratory Bioreactor Engineering of East China University of Science and Technology, Shanghai 200237, China; 2Shanghai Biomodel Organism Technological Development Company Ltd., Shanghai 201203, China; 3Shanghai
Abstract: Onconase， a ribonuclease firstly extracted from oocytes of frog (Rana pipiens)， displays significant anti-tumor effects against many kinds of tumor cell lines in vivo and in vitro. We have expressed and purified the recombinant Onc in E.coli. The recombinant protein has similar activity as its natural form previously reported. The IC50 of the recombinant protein on B16 cell was tested and its anti-tumor activity was studied on the B16 melanoma animal model. The results showed that Onc has a pronounced effect on B16 cell (IC50 = 6.37 μmol/L)， and greatly inhibits the growth of tumor cells and retard the mice's death. The experiment offers an efficient simple method to produce the recombinant Onc protein with similar activity as the natural one， and enriches the knowledge of Onc for its anti-tumor role and provides some clues for melanoma therapy.
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