The Application of Genome Editing in the Construction of Anti-Apoptotic Engineering Cells

Abstract: Since the 21st century， people have made great progress in the field of biotechnology. The advent of genome editing technology brings possibility to precisely modify target genes， which means we can perform certain tasks in living cells like inserting， deleting， replacing， activating or suppressing specific DNA fragments. Furthermore， more and more new recombinant protein drugs have been developed for combating severe diseases such as cancers. Compared with traditional chemical drugs， it has significant advantages such as high specificity and low side effects. Besides， its therapeutic effect has been widely recognized. Currently， the engineering cell lines used for the production of therapeutic recombinant proteins are mainly derived from mammal cells. However， due to various environmental factors in the production process， the apoptosis rate of engineering cell is extremely high， which seriously affects the expression level of the proteins， resulting in greatly increasing the production costs. After clarifying the mechanisms involved in the growth and death of cells， researchers， by utilizing genome editing technology， modified cells to improve the expression level. Here we attempt to summarize the research results in this field.

CSTR: 32200.14.cjcb.2018.12.0011