Comparative Analysis of the Codon Preference Patterns in Two Species of Camellia sinensis Based on Genome Data

Abstract: Analysis of codon preference patterns is helpful to improve the expression level of exogenous genes and explore the genetic evolution laws. In this paper， CodonW 1.4.2， SPSS 22.0 software and Perl， Python languages were used to systematically analyze the CDS sequences of genomes of the two species of Camellia sinensis， thus the pattern of codon preference and the high frequency codons were obtained， the source of codon usage variation were determined. The results showed that the codon preference of genomes of the two species of Camellia sinensis was mainly affected by mutation pressure， natural selection and other factors to some extent. It showed that the genomes of two species of Camellia sinensis preferred to use codons ending in A/T， and they had high similarity in codon usage patterns. It showed that heterologous expression receptors of Camellia sinensis var. assamica (CSA) genes were preferentially selected Arabidopsis thaliana， Poplar and Tobacco， but Camellia sinensis var. assmica (CSS) genes were preferentially selected Poplar. These results provide an important basis for analyzing the evolution of two species of Camellia sinensis and improving the efficiency of gene heterologous expression.

CSTR: 32200.14.cjcb.2018.12.0007