Home > Browse Issues > Vol.40 No.11
The Construction Strategy and Progress of the Recombinant Antibody Expression Vector in CHO Cells
Li Yanmei1,2, Tian Zhengwei1,2, Xu Danhua1,2, Wang Wen1,2, Wang Xiaoyin1,2, Zhang Guihu3, Wang Tianyun1,2*
1Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang 453003, China; 2International Joint Research Laboratory for Recombiant Pharmaceutical Protein Expression System of Henan, Xinxiang 453003, China; 3Electric Power Bureau Staff Hospital of Pingdingshan, Pingdingshan 467000, China
Abstract: In recent years， recombinant proteins are used in the treatment of different diseases. Among of them， monoclonal antibodies (mAbs) are currently the fastest growing class of biotherapeutic recombinant proteins. Chinese hamster ovary (CHO) cell is the most commonly host cells for the production of recombinant antibodies. Expression vector is an important part of recombinant antibody expression， which influences the expression level and quality of recombinant antibody. At present， the construction strategies of recombinant monoclonal antibodies expression vectors are mainly multiple-promoter expression vector， IRES-mediated vector， Furin-2A-mediated vector and antibody fusion protein in CHO cells. Among of them， Furin-2A-mediated vector present an effective approach to construct the recombinant protein expression vector with a variety of advantages， especially of its high “selfcleavage” efficiency， good balance of two genes expression and its short gene sequences. In this study， the strategies of constructing recombinant monoclonal antibodies expression vector in CHO cells and its potential advantages， disadvantages and progress are reviewed.
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