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Study of Cannabinoid Receptor 1 Mediated Monocytes/Macrophages M1 Polarization via PI3K/AKT Signaling Pathway
Yang Lin1,2, Tian Lei1,2, Chang Na1,2, Duan Xianghui1,2, Li Liying1,2*
1Department of Cell Biology, Capital Medical University, Beijing 100069, China; 2Municipal Laboratory for ‘Liver Protection and Regulation of Regeneration’ , Beijing 100069, China
Abstract: RT-qPCR， Western blot， flurescence-activated cell sorting (FACS) were employed to detect whether cannabinoid receptor 1 (CB1) was involved in bone marrow monocytes/macrophage (BMMs) M1 polarization. The results showed that ACEA (CB1 agonist， 1 μmol/L) promoted the mRNA levels of M1 type macrophage gene signatures (CD86， IL-1， MIP-1β， NOS2， IL-6， TNF-α) in BMMs. The protein level of CD86 deteced by FACS were increased in BMMs induced by ACEA. When BMMs were pretreated with LY294002 (specific PI3K/AKT signal pathway inhibitor)， ACEA-induced (1 μmol/L) increases of M1 gene signatures mRNA levels was suppressed. Furthermore， Western blot analysis showed the protein level of phosphorylated AKT (p-AKT) was increased in ACEA-treated BMMs. When BMMs were pretreated with AM281 (CB1 antagonist， 1 μmol/L)， p-AKT protein level was inhibited. The result showed that CB1 mediated monocyte/macrophage M1 polarization via PI3K/AKT signaling pathway.
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