PML Represses C-Myc Expression through Transcriptional Regulation of CTCF

Abstract: This study investigated the effect of promyelocytic leukemia protein (PML) on transcription regulation of CCCTC-binding factor (CTCF) and its downstream target gene C-Myc. The expression levels of PML and CTCF in clinical samples were analyzed by using fluorescence quantitative PCR， and the correlation between them was also analyzed. PML and CTCF eukaryotic expression vectors were constructed， and the effect of PML on CTCF expression level was analyzed by fluorescence quantitative PCR and Western blot in co-transfected cells. The regulation of PML on CTCF promoter region was analyzed through the Dual-Luciferase reporter gene system， and the influence of PML on the expression level of C-Myc downstream target gene of CTCF. It showed that there was a negative correlation between the expression of PML and CTCF in children ALL. PML reduced the expression of CTCF by inhibiting the transcription of CTCF and interfered with the regulation of the CTCF downstream target gene C-Myc. At the same time， there may be a binding region of PML in the promoter region of CTCF， resulting in inhibition of CTCF promoter activity.

CSTR: 32200.14.cjcb.2018.11.0006