Home > Browse Issues > Vol.40 No.11
The Application of Antioxidant on Removing Reactive Oxygen Species and Enhancing Agrobacterium tumefaciens Mediated Transformation Efficiency
Zhong Xuanbo, He Mengdi, Zhou Qizheng, Wang Guifeng, Tang Guixiang*
College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
Abstract: The browning and necrosis of plant cell and tissues， shoot escape and recalcitrant are three major factors to influence transformation efficiency in the Agrobacterium-mediated transformation process in many plant species. Recent studies had shown that these phenomena were closely related to reactive oxygen species (ROS). Excessive ROS could affect the plant cell totipotency. Addition antioxidants in the tissue culture media could reduce the explant tissue and cells necrosis during Agrobacterium-mediated transformation process， improve the Agrobacterium vitality and promote the explant tissue and cells regeneration. This paper reviewed the ROS production during Agrobacterium- mediated transformation and its effect on the transformation efficiency， especially the role of new type of antioxidant―lipoic acid to enhance transformation efficiency during Agrobacterium-mediated transformation.
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