Characterization of M1 Macrophage Model Prepared from Normal Human Monocyte SC Cells

Abstract: We used phorbol 12-myristate 13-acetate (PMA) to induce the human normal monocyte SC cells to differentiate into SC-macrophages. To address whether SC cells could differentiate into typical M1 macrophages， we analyzed morphology， endocytosis， surface markers and secretion of inflammatory cytokines. We found that SC-macrophages grew in adherence mode， and their morphology was round or oval in general， while a small portion of cells were found to have elongating or irregular morphology. SC-macrophages could engulf fluorescent microspheres primarily via caveolae related endocytosis. SC-macrophages surface marker CD11b and LPS receptor CD14 were found to be significantly up-regulated as compared with SC cells; while post-LPS stimulation， SCmacrophages showed activation morphologies， including cell elongation， protrusion formation; the maturation marker of CD80 and CD86 were found to be upregulated， along with the significantly up-regulated secretion of inflammatory cytokines of TNF-α， IL-1β， IL-6 and IL-8， which was consistent with the qPCR analysis on these cytokines. SC-macrophages had normal M1 macrophage phenotypes， and our evaluation favors the wide application of this cellular model in the field of macrophage associated investigations.

CSTR: 32200.14.cjcb.2018.11.0013