Gene Clone and Expression Analysis of Sucrose Transporter Gene Family from Vegetable Soybean

Abstract: Sucrose is the main or even the only form of long distance transport of carbohydrates in higher plants， and sucrose transporter plays a very important role in the process of sucrose transport. In this study， 9 sucrose transporter genes were cloned from vegetable soybean Mindou 6， named GmSUT-1-GmSUT-9， and their bioinformatics， phylogeny and tissue expression were analyzed. The results showed that GmSUTs belonged to MFS superfamily. According to the SUT phylogenetic analysis， GmSUTs was divided into three subgroups of SUT1， SUT2 and SUT4， of which GmSUT-1， GmSUT-2， GmSUT-4， GmSUT-5， GmSUT-6， GmSUT-7 and GmSUT-8 belonged to subgroup SUT1， GmSUT-9 belonged to subgroup SUT2 and GmSUT-3 belonged to subgroup SUT4. The differences in amino acid sequences of members of SUTs subgroups might lead to different roles in transmembrane transport of sucrose， and their expression patterns and physiological functions were also different. The total expression of 7 genes in the SUT1 subfamily of soybean is similar， the highest expression is in the flower， while the expression of the seeds， leaves， stems and roots is relatively low， and the expression of GmSUT-3 in SUT4 subgroup and GmSUT-9 in SUT2 subgroup was relatively low. This study preliminarily clarified the structure and tissue expression characteristics of the sugar transporter family gene in vegetable soybean， which laid the foundation for the further development of sucrose transporter gene regulation in the accumulation and distribution of sucrose in the development of vegetable soybean seeds.

CSTR: 32200.14.cjcb.2018.11.0010