Intervention of Aerobic Exercise and Lycium ruthenicum Murr Flavone on Mice with Depression and Differential Expression of TLR4/MyD88/NF-κB Related Factors
    

Abstract: This work was aim to investigate the antidepressant effect of aerobic swimming (SW) and Lycium ruthenicum murr flavones (LRMF) on depressed mice and the differential expression of TLR4/MyD88/NF- κB related factors in brain and blood. Fifty male KM mice were used to construct a depression model， which was administered by swimming and/or black flavonoids (200 mg/kg). The behavior of mice， the morphological changes of brain tissue and the expression of related factors proteins and mRNA under the intervention of aerobic exercise and flavonoids were tested by behavioral assessment， ELISA， brain tissue Nissl staining， immunohistochemistry (IHC) and qRT-PCR. The results of behavioral evaluation showed that the CUMS group had a very significant differences in behavioral evaluation compared with the control group after modeling (P<0.01). After intervention， intervention group had different degrees of improvement in behavioral evaluation compared with group M (P<0.05， P<0.01). The contents of 5-HT (5-hydroxytryptamine)， NE (norepinephrine) and DA (dopamine) in the brain tissue of the intervention group were higher than those in the M group， and the LRMF+SW was highest (P<0.01)， followed by the SW group. The results of microscopic observation showed that the neurons in the model group had the highest degree of injury， nuclear fixation， deep stained and cavitation， and the LRMF+SW group had the best intervention effect. IHC showed that the expressions of TLR4， MyD88， NF-κB and IL-1β protein in the intervention group were lower than those in M group (P<0.05， P<0.01)， and the expression level of LRM+SW group protein was the lowest (P<0.01)， followed by the LRMF group. The results of qRT-PCR showed that the expression levels of TLR4 and MyD88 in brain tissues were higher than those in blood tissues (P<0.05)， and they were positively correlated. Compared with group M， the expressions of TLR4， MyD88， NF-κB and IL-1β mRNA in LRMF+SW group were the lowest (P<0.01)， and SW group was lower than those in LRMF group (P<0.05). The results showed aerobic swimming and dark fruit flavonoids could relieve or treat brain damage caused by depression in mice， enhance immunity and neuronal repair， and the combined intervention had the best effect. The mechanism may be through antagonizing TLR4 inhibition MyD88， anti-inflammatory gene activation， TLR4， MyD88 in the brain tissue and blood tissue there is a moderate correlation. As an early detection method， the detection of circulated RNA in plasma is a good clinical prospect for the diagnosis and monitoring of brain injury caused by depression.

CSTR: 32200.14.cjcb.2018.11.0007