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Construction of Hydrogen Sulfide Producing Enzyme LCD Mutants in Chinese Cabbage Based on CRISPR/Cas9 Technology
Ma Xiaoli1,2#, Jing Qiaoli1#, Pei Yanxi1*
1School of Life Science, Shanxi University, Taiyuan 030006, China; 2College of Biological Science and Technology, Jinzhong University, Jinzhong 030600, China
Abstract: Hydrogen sulfide (H2S) is an important gasotransmitter in plant cells and plays an important role in regulating plant growth， development and stress resistance. L-cysteine desulfydrase (LCD) is the key enzyme to produce endogenous H2S. Chinese cabbage (Brassica campestris) is one of the main vegetable crops in northern China. In order to study the physiological function of H2S signal in Chinese cabbage and provide new materials for breeding， the CRISPR/Cas9 gene editing technique was used to knock out the LCD gene. Gene editing vector pYAO-LCD was transformed into ‘Zhongbai 60’ by genetic transformation mediated by Agrobacterium tumefaciens (LBA4404)， and 4 plants of genetic transformation were obtained by gene editing. DNA sequencing showed that the target site was correspondingly edited. The endogenous H2S content in different editors decreased to varying degrees.
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