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Establishment and Biological Characteristics of Lung Epithelial Cell Line MFLE-001 from Macaca fascicularis
Song Qian1, Sun Hua1, Chen Yan1, Tang Xinhua2, Cheng Xianshuo1, Yuan Lu1, Cong Li3, Xu Liangzhi3, Luo Fagao3, Dong Jian1*
1Yunnan Cancer Hospital & The Third Affiliated Hospital of Kunming Medical University Cancer Institute, Kunming 650118, China; 2The First People’s Hospital of Yunnan Province, Kunming 650000, China; 3Yunnan Yingmao Biotechnology Co., Ltd., Kunming 650000, China
Abstract: In the present study， the lung epithelial cell line from Macaca fascicularis was established and its biological characteristics was identified. The cells derived from a pulmonary tissue of Macaca fascicularis were primarily cultured and purified by differential attachment technique. The biological characteristics of the cell line were studied by using optical microscope， electron microscopes， growth curve， karyotype analysis and lung epithelial marker. The results showed that the growth of cells were stable and uniform in vitro. The cell doubling time was 4.8 d and the results of immunohistochemistry showed the expressions of CK and CK-7. The vimentin was positive and the chromosome numbers were 2n=42， all of which were accorded with the characteristics of lung epithelial cell line from Macaca fascicularis. MFLE-001 has been proved to be the first lung epithelial cell line of Macaca fascicularis and is an ideal experimental model for lung disease research and drug screening.
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