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Phyllostachys edulis LTR Transposon-Cloning and Transcriptional Activity Identification of PHRE6
Zhang Zanyi1,2, Zhou Mingbing1,2*, Tang Dingqin1,2
1State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China; 2Zhejiang Provincial Collaborative Innovation Center for Bamboo Resources and High-Efficiency Utilization, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China
Abstract: Long terminal repeats (LTR) retrotransposons are a type of mobile DNA sequence that is commonly found in eukaryotic genomes and are named after having long terminal repeats at both ends. Most LTR retrotransposons are able to sense changes in the external environment， with transcriptional activation and transposition activation properties. In this study， a complete LTR retrotransposon is cloned from the genome of Phyllostachys edulis (Ph. edulis)， named PHRE6 (Phyllostachys edulis retrotransposons 6). The total length of the transposon is 5 620 bp which has GAG and POL conserved domains. The transcriptional changes of PHRE6 in DNA methylation inhibitors and the treatment of different stress treatments (including radiation， high temperature， low temperature and high salt) in Phyllostachys edulis seedlings are detected by Real-time fluorescence quantitative PCR and after DNA methylation inhibitor treatment. High temperature (42 °C)， low temperature (16°C， 4 °C)， high salt (100 mmol/L， 200 mmol/L， 300 mmol/L NaCl solution) stress levels significantly increase the expression level. This result indicates that PHRE6 is a transcriptionally active retrotransposon and may be involved in the response process of Ph. edulis.
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