Home > Browse Issues > Vol.40 No.8
Effects of Mirtron MicroRNA6894-5p Overexpression on Migration， Invasion and Proliferation of Human Gastric Carcinoma Cells
Zhao Maojia, Peng Meixi, Qin Yilu, Liu Shuiqing, Chen Yanlin, Yang Liping, Liu Manran*
Key Laboratory of Laboratory Medical Diagnostics, Chinese Ministry of Education, Chongqing Medical University, Chongqing 400016, China
Abstract: This article was to investigate the effects of human microRNA6894-5p (miRNA6894-5p) on migration， invasion and proliferation of human gastric carcinoma cells. Mimics of miRNA6894-5p were constructed and transfected into gastric cancer cells. The expression of miRNA6894-5p in gastric carcinoma cells after transfection was determined by quantitative real time PCR (qRT-PCR). The migration， invasion and proliferation ability of gastric cancer cells were detected by Transwell chamber test and Cell Counting Kit-8 assays respectively. Luciferase reporter gene assay was used to detect the targeted relationship of miRNA6894- 5p and hepatic protein A3. The mRNA and protein expression of hepatic protein A3 were detected by qRT-PCR and Western blot. The results showed that microRNA6894-5p mimics were successfully transfected into gastric carcinoma cells. Compared with control cells， the ability of migration and invasion were significantly increased in overexpression cells (P<0.05) and miRNA6894-5p overexpression could significantly promote gastric cancer cells proliferation (P<0.05). The relationship between miRNA6894-5p and EFNA3 was confirmed by Luciferase reporter gene assay. The mRNA and protein expression of EFNA3 were downregulated in miRNA6894-5p overexpression cell. The above results indicate that miRNA6894-5p can promote the migration， invasion and proliferation of gastric cancer cells.
CN
EN