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Identification of Human COX7A2L Genotypes and Their Effects on Mitochondrial Function and Assembly of Supercomplex
Ge Linghong1, Li Wenna1, Huang Jiatao1, Lü Jianxin1,2*
1School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou 325035, China; 2Hangzhou Medical College, Hangzhou 310053, China
Abstract: COX7A2L is a similar protein of the mitochondrial respiratory enzyme COX subunit 7a. Two mouse strain specific COX7A2L isoforms differentially affect mitochondrial function， however， the regulatory role of COX7A2L in human mitochondrial function is not known. Here， we find that COX7A2L is expressed as a single isoform in human cells and located in the mitochondrial inner membrane. The sequence of COX7A2L is highly conserved， since no polymorphic sites are detected in 96 human subjects. Furthermore， we find both mitochondrial respiratory function and ATP content are decreased in COX7A2L knockout 293T cells compared with wide type 293T cells. Although our results indicate that loss of human COX7A2L inhibits the assembly of supercomplex III+IV， supercomplex In+IIIn+IVn assembly is differently affected， suggesting that supercomplex In+IIIn+IVn assembly is not completely dependent on the expression of COX7A2L. In summary， our data indicate that human COX7A2L is more conserved and plays a role like mouse long isoform of COX7A2L.
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