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Human Amniotic Epithelial Stem Cells-Derived Exosomes Promote Neuronal Differentiation of Mouse Neural Stem Cells
Zhang Jiaofei1, Lin Jianhua2, Wang You2, Xu Huiming3*, Zhang Qianjun1,4*
1Institute of Reproduction and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha 410078, China; 2Department of Obstetrics and Gynecology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China; 3Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China; 4National Engineering Research Center of Human Stem Cells, Changsha 410205, China
Abstract: Human amniotic epithelial stem cells (hAECs) have been reported to promote the regeneration of damaged neurons and the restoration of neurologic function， but whether hAECs can promote the neuronal differentiation of neural stem cells (NSCs) is rarely mentioned. In this present study， they found that hAECs could promote the neuronal differentiation of mouse NSCs (mNSCs). Additionally， stem cells-derived exosomes (Exos) can remain some characteristics of stem cells. They determined to investigate the effect of hAECs-derived exosomes (hAECs-Exos) on the neuronal differentiation of mNSCs. Firstly， hAECs-Exos were extracted by ultracentrifugation. Then， hAECs-Exos at various concentrate were cocultured with mNSCs. The results suggested that the group with 200 ng/mL hAECs-Exos could significantly promote the neuronal differentiation of mNSCs compared with the control group with no hAECs-Exos， as demonstrated by the higher percentages of NeuN positive neurons derived from mNSCs. We hypothesized that the hAECs-Exos might retain some characteristics of hAECs. It provided a suitable microenvironment for the neuronal differentiation of mNSCs attributing to neurotrophic factors， growth factors， miRNAs and other active components. This could facilitate the neuronal differentiation of endogenous or exogenous NSCs， and thus contributed to the regeneration of damaged or degenerated neurons. This study will provide a preclinical study for the hAECs-Exos in regenerative medicine.
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