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Study on Morphology of Skeletal Muscle Fiber and Expression of Muscle Shrinkage Protein in Rats after Treatment with Soybean Isoflavone
Tang Yi1, Tan Jinlong1, Luo Qihui1,2, Huang Chao1, Liu Wentao1,2, Chen Zhengli1,2*
1Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; 2Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
Abstract: To investigate the effect of SIF on muscle growth， they used 6-week-old SD male rats as test subjects. We used hematoxylin-eosin staining， immunohistochemistry， and Real-time fluorescence quantitative PCR to study morphological changes of myofibers and changes in the expression of α-actin， myosin and mRNA in male rats after treatment with different concentrations of soybean isoflavone. The results of the study showed that the muscle fiber diameters of the middle and high dose groups were significantly higher than those of the vehicle control group (P<0.01). Immunohistochemical results showed that the expression of α-actin was significantly higher in the medium and high dose groups than in the vehicle control group (P<0.01); the high dose group of myosin was significantly higher than the vehicle control group (P<0.01)， and the middle dose group was significantly higher than the vehicle control group (P<0.05). The results of real-time fluorescence quantitative PCR showed that the expression levels of target gene α-actin and myosin mRNA were basically consistent with the protein expression. The results showed that soybean isoflavones could thicken the muscle fibers by promoting the expression of actin， myosin and their mRNA in muscle， which could provide theoretical guidance and experimental basis for soybean isoflavone to improve muscle contraction and promote skeletal muscle fiber thickening.
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