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The Effect of Calcium Signaling Pathway on the Expression of Genes Regulated by crz1 in Saccharomyces cerevisiae
Lu Yiting, Wang Jingya, Zhao Yunying*, Deng Yu*
School of Biotechnology, the National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China
Abstract: The binding site of the transcription factor Crz1 (GNG GC[T/G] CA or GNG GCT G) could be found in the promoter regions of 24 genes by analyzing the promoter sequence of 120 calcium-sensitivity genes in Saccharomyces cerevisiae. In this study， the effects of calcium signal pathway on the expression levels and subcellular localizations of these 24 genes have been examined. The results indicated that the specific expressions of 19 genes and the subcellular localizations of the proteins encoded by these genes could be induced by calcium signaling pathway through the transcription factor Crz1. We found that the functions of five genes were involved in metabolism (TPS1， PHO86， ERG3， ARG82 and AKR1)， five genes were involved ion homeostasis (CSG2， PMC1， VMA10， MNR2 and VAM2)， four genes were functionally related to protein sorting (PEP3， VPS36， VPS27 and VPS4) and five genes were functionally related to transcription (DEP1， IMP2′， THP1， SGF29 and ROX3). The results of this study have provided theoretical basis for studying the regulation mechanism of calcium homeostasis in Saccharomyces cerevisiae.
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