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Long Non-Coding RNA PVT1 Promotes the Proliferation and Migration of Gastric Cancer Cells
Zhang Xiaotian1*, Huang Saiya1, Wang Qian2, Liu Wei1
1Department of Medical Laboratory Science, Fenyang College of Shanxi Medical University, Fenyang 032200, China; 2Department of Medical Imaging, Shanxi Medical University, Taiyuan 030001, China
Abstract: In this study， the effect of long non-coding RNA PVT1 on the proliferation and migration of gastric cancer cells were investigated. The expression level of PVT1 in gastric cancer cells MGC-803， HGC-27 and GES-1 were detected by qRT-PCR. Techniques of over-expression and RNA interference were applied to increase and decrease the level of LncRNA PVT1， the abilitiy of proliferation was examined by CCK-8 assay. The cell cycle was determined by flow cytometry. The ability of migration was detected using wound healing and Transwell assay. The protein expression of p21 and E-cadherin were determined by Western blot and qRTPCR. The expression of LncRNA PVT1 in gastric cancer cells was higher than that in gastric mucosal cell (P<0.01). The migration and proliferation of the MGC-803 cells were eleva ted after the level of LncRNA PVT1 was over-expressed， meanwhile， the level of p21 and E-cadherin were reduced (P<0.01). In contrast， the migration and proliferation of the HGC-27 cells were reduced after the level of LncRNA PVT1 was silenced， the expression of p21 and E-cadherin were increased after the silencing of LncRNA PVT1 (P<0.01). These results in dicated that the expression of LncRNA PVT1 was up-regulated in gastric cancer cells， and the LncRNA PVT1 promoted the proliferation and migration of the gastric cancer cells. The protein of p21 and E-cadherin might play an important role in this regulation.
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