Cytoskeletal Rearrangement and Proliferation Inhibition in Wangzaozin A-Treated HeLa Cells

Abstract: The effects of wangzaozin A on cellular proliferation inhibition and the cytoskeleton rearrangement of HeLa cells were investigated by using typan blue exclusion， Giemsa staining， flow cytometry and immunofluorescence assay. The results showed that 0.8-2.0 μmol/L wangzaozin A inhibited the proliferation of HeLa cells， inducing G1 arrest and obvious changes of cellular morphology. The number of cells with asymmetric long strips increased， which was similar to effect of cytoskeleton inhibitors. Wangzaozin A could obviously alter the arrangement direction of microtubule and keratin filaments， and induced the polymerization of them， significantly increased in their mean fluorescence intensity. But it decreased number of stress filaments， and markedly reduced mean fluorescence intensity. These effects were shown concentration- and time-dependent. This phenomenon is similar to the effects of paclitaxel on HeLa cells. Western blot assays suggested that wangzaozin A had on obvious effect on the contents of keratin， β-actin and α-tubulin in HeLa cells， indicating that the changes in the amount of cytokeleton filaments induced by wangzaozin A were not related to the amount of monomeric protein. These results suggested that cytoskeletal rearrangement induced by wangzaozin A severely interfered with cellular homeostasis， leading to significant changes in cell and nuclear morphology and inhibition proliferation of HeLa cells. However， the direct target for cytoskeleton rearrangement induced by this compound still needs further study.

CSTR: 32200.14.cjcb.2018.07.0012