miR-143 Enhances Drug Sensitivity of Cytarabine in SKM-1 Cells

Abstract: The aim of this study was to investigate whether miR-143 could enhance the drug sensitivity of cytarabine (Ara-C) in SKM-1 cells and the corresponding mechanism. The CCK-8 method was used to screen out the best conditions for Ara-C intervention. The cell cycle and apoptosis rate were measured by flow cytometry. Western blot was used to detect the expression of Akt and pAkt protein. The results showed that the LV-hsa-miR-143+Ara-C group was significantly higher (88.50%) than the LV-control+Ara-C group (67.47%) and LV-hsa-miR-143 (31.01%) (P<0.05). The percentage of cells in G1 phase of LV-hsa-miR-143+Ara-C group (87.24±6.12)% was significantly higher than that in LVcontrol+ Ara-C group (72.10±3.71)% and LV-hsa-miR-143 group (57.73±5.02)%， showing obvious G1 arrest. The relative expression of pAkt protein in LV-hsa-miR-143+Ara-C group was significantly lower than that in LV-control+Ara-C group and LV-hsa-miR-143 group (P<0.05). There was no significant differences in the relative expression of Akt protein in each group (P>0.05). The above results indicate that overexpression of miR-143 can increase the drug sensitivity of cytarabine in SKM-1 cells. The mechanism may be related to the decrease of Akt phosphorylation.

CSTR: 32200.14.cjcb.2018.07.0011