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Bone Morphogenetic Protein (BMP9) Attenuate the Lipid Metabolism and Lipid Utilization in Breast Cancer Cells through AMPK Signal Pathway
Xia Jing, Gou Liyao, Liu Mengyao, Wan Qun, Tang Min, Sun Shilei, Zhang Yan*
Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China
Abstract: To investigate the effect of bone morphogenetic protein 9 (BMP9) on lipid metabolism in breast cancer， our group set up an Ad-Easy system to overexpress BMP9 in breast cancer cells. After the overexpressed of BMP9， key factors on lipid de novo synthesis， degration and transfer of lipid in breast cancer cells were detected by q-PCR whilst lipid content variation was measured by NEFA measure kit. Moreover， the activity of AMPK signal pathway was detected by Western blot. The efficiency of AMPK on BMP9 was proved by supplying inhibitor of AMPK in the culture. These key factors on breast cancer cells were detected by q-PCR. Concentration of free fatty acid (FFA) in the medium was measured by NEFA measurement kit， which was detected after cell culture and treatment. The result showed that the expression levels of key factors in lipid metabolism on breast cancer cells were reduced by BMP9 (P<0.05)， but hormone sensitive lipase (HSL) increased. Content of lipid in breast cancer cells decreased in both MDA-MB-231 and MCF-7 cells. Our study showed BMP9 might attenuate the expression of key factors on lipid metabolism and utilization of breast cancer cells through AMPK signal pathway.
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