CircRNA circTCF25 Regulates the Expression of CDK6 by miR-103a-3p and miR-107 to Promote the Proliferation and Migration of Bladder Cancer

Abstract: The aim of the present study is to investigate the effect of circRNA circTCF25 on proliferation and migration of bladder cancer. The expression of miR-103a-3p and miR-107 in cancer tissues and adjacent noncancer tissues was determined by RT-qPCR. The expression of CDK6 in bladder cancer cell lines and cancer tissues and their adjacent non-cancer tissues was examined by Western blot and immunohistochemical staining. The circTCF25 over-expression vector was transfected into two kinds of bladder cancer cell lines， and the expression of circTCF25， miR-103a-3p and miR-107 were determined by RT-qPCR. Dual luciferase reporter gene assay was used to verify the combination of circTCF25 and miR-107 and the target gene CDK6 of miR-107. The abilities of migration and proliferation of the bladder cancer cell lines were determined by wound healing assay and Edu assay， respectively. As a result， lower expression of miR-103a-3p and miR-107， while higher expression of CDK6 was found in bladder cancer tissues compared to their adjacent non-cancer tissues. The expression of circTCF25 in the group transfected with over-expression vector was higher than control group. After up regulation of circTCF25， the expression of miR-103a-3p and miR-107 were decreased， while the protein level of CDK6 was increased. Dual luciferase reporter gene assay showed that circTCF25 could directly bind to miR-107 and reduce the inhibition of target gene CDK6. And the capabilities of proliferation and migration of bladder cancer cell lines were increased after over expression of circTCF25. Taken together， circTCF25 may promote the proliferation and migration of bladder cancer through miR-103a-3p/miR-107-CDK6 axes.

CSTR: 32200.14.cjcb.2018.06.0010