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Cloning and Expression Analysis of Lipoxygenase Gene AmLOX1 from Snapdragon
Wang Shaojie1, Liu Xiaohui1,2, Hu Zenghui1, Leng Pingsheng1*
1School of Landscape Architecture, Beijing University of Agricuture, Beijing 102206, China; 2Beijing Florascape Co. Ltd, Beijing 102206, China
Abstract: Lipoxygenase (LOX) is a key enzyme in jasmonic acid signaling pathway. In this study， the LOX gene of snapdragon was cloned and named as AmLOX1. The open reading frame of the AmLOX is 2 649 bp， which encodes 883 amino acids. The theoretical isoelectric point of AmLOX1 protein is pH6.06， and the relative molecular mass was 100.52 kDa. AmLOX1 shares high homology with the LOX genes of other plants. The Realtime fluorescence quantitative PCR analysis was used to analyze the relative expression of AmLOX1 in snapdragon. The expression of AmLOX1 in snapdragon flower was significant higher than that of root， stem or leaf. While， it expressed highest in lower pedals around the floral organs. And the relative expression level of the decline period was the highest among different developmental stages.
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