Exploration for the Toxicity of TPL-PEI-CyD to Hepatocytes and Effects on Breast Cancer Stem Cells

Abstract: The purpose of this study was to survey the effects of TPL-PEI-CyD on breast carcinoma stem cells. The cytotoxicity of TPL-PEI-CyD and TPL on HL-7702 was compared by CCK-8. After incubation and culturing by TGF-β1， the MCF-7 cells were assessed by flow cytometry for the proportion of CD44 + CD24 – cells; Then， sorted the CD44 +CD24 – cells by magnetic-activated cell sorting as MCF-7 stem cells. MCF-7 stem cells were dosed by TPL-PEICyD and then assessed by double staining flow cytometry for the variation of CD44 +CD24 – proportion. Compared with TPL， the toxicity of TPL-PEI-CyD on liver cells was significantly declined (P<0.05). Breast carcinoma stem cells could be enriched by TGF-β1 and isolated from MCF-7 cells with immunomagnetic sorting. After dosed with TPL-PEI-CyD， the proportion of CD44 +CD24 – in MCF-7 stem cells was significantly lower than dosed with TPL (P<0.05). TPL-PEICyD could effectively suppress the property of breast carcinoma stem cell. It showed the unique effect of traditional Chinese medicine as a efficient and low toxic drug carrier complex for breast carcinoma treatment.

CSTR: 32200.14.cjcb.2018.06.0006