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Isolation， Identification and Analysis of ORF2 Gene Evolution of Porcine Circovirus Type 2 in Shandong Province
Peng Tao1,2,3#, Ma Zicheng1,2,3#, Zhang Libin4, Li Baoquan1,2,3* , Wang Fangkun1,2,3
1Department of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China; 2Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Taian 271018, China; 3Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Taian 271018, China; 4New Hope Liuhe Co., Ltd, Weifang 261000, China
Abstract: In this study， we made a pathological diagnosis of swine which suspected of suffering from postweaning multisystemic wasting syndrome (PMWS) in one swine farm in Shandong Province， by pathological examination and histopathological section observation. Then， PK15 cells were inoculated with the samples that were positive for PCV2 by PCR， and a strain of PCV2 (SDWF20107) was isolated by continous subculture. The results of similarity analysis of the ORF2 gene showed that the nucleotide similarity between this strain and other reference strains ranged from 77.2% to 99.6%， and the amino acid similarity ranged from 82.1% to 99.6%. Phylogenetic analysis based on ORF2 genes indicated that the isolated strain belongs to the 2.1d subtype. According to the characteristic of the epidemic strain， the swine farm switched to a highly efficientive and adjusted the immune program. As a result， the symptoms of the diseased swine soon disappeared and the normal production levels were restored. The present study provided reference data for the genotyping and variation of PCV2 in swine farms， and provided theoretical basis for future epidemiological studies and prevention and control of PMWS.
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