Effects of ME3 on the Abilities of Proliferation， Migration， Invasion and Mesenchymal Transition in Human Glioma Cells

Abstract: The purpose of this study is to investigate the effects of malic enzyme 3 (ME3) on the abilities of proliferation， migration， invasion and mesenchymal transition in human glioma cells. We first detected the relative levels of mRNA and protein of ME3 in different glioma cell lines， respectively. Then the specific plasmids (sh-ME3) were transfected into the glioma cells U87MG and U251MG. The effects of ME3 on cell proliferation and colony formation were detected by CCK-8 assay and clone formation assay， respectively. Transwell assays were used to examine the ability of ME3 on cell migration and invasion， and wound healing assay was also used to measure the cell migration. Western blot was used to examine the effect on the mesenchymal transition of glioma cells with the ME3 knockdown. The results showed that the ME3 knockdown decreased the ability of proliferation， migration and invasion in U87MG and U251MG cells， and also inhibited the expression of mesenchymal markers and mesenchymal transition of the glioma cells. Collectively， the present study demonstrates that ME3 plays a critical role in the development of glioma and may provide a potential therapeutic target for it.

CSTR: 32200.14.cjcb.2018.06.0002