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The Effect of All-Trans Retinoic Acid on BMP9 Induced Mature Differentiation of Hepatic Progenitor Cells
Gong Mengjia1, Hui Hui1, Cui Jiejie1, Bi Yang1, He Yun1,2*
1Stem Cell Biology and Therapy Laboratory of the Children’s Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China; 2Department of Gastroenterology Pediatric Surgery of the Children’s Hospital of Chongqing Medical University, Chongqing 400014, China
Abstract: This study was to investigate the effect of ATRA on Ad-BMP9 induced mature differentiation of hepatic progenitor cells (HP14-19). Ad-BMP9 and ATRA induced the differentiation of HP14-19， individually or jointly. Luciferase reporter gene to detect the expression of ALB-Gluc. Real-time PCR detected the level of mRNA of liver related genes and protein level was detected by Western blot and immunofluorescence. PAS staining and ICG uptake assay were used to detect the function of mature differentiation. Ad-BMP9+ATRA group could promote the differentiation. The activity of ALB-Gluc， mRNA expression level of ALB and TAT， and protein levels of ALB， CK18 and UGT1A were higher than that of Ad-BMP9 group and ATRA group. The levels of DLK and AFP were decreased (P<0.05)， liver function improved significantly. ATRA and Ad-BMP9 induced the mature differentiation of HP14-19， and the combined effect stronger than that of the single effect.
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