Home > Browse Issues > Vol.40 No.5
Effect of Colon Cancer Microenvironment on the Morphology， Proliferation， Cycle， CD34 and CD90 of HMSC-bm
Zhao Huiqiao, Lu Nianhua, Zhang Xudong, Liu Na, Liu Xuefeng, Chen Zhengjun, Jing Ming*
Gansu University of Chinese Medicine, Lanzhou 730000, China
Abstract: The purpose of this study was to investigate the effects of different conditions of colon cancer microenvironment on human mesenchymal stem cells-bone marrow (HMSC-bm). In the experiments， the different ratios of HMSC-bm and the colon cancer cell SW480 were non-contact co-cultured with Transwell for 3 d， 5 d and 7 d. The microscopic morphology， proliferative capacity， cell cycle and cell surface marker molecules CD34 and CD90 of each group were tested to investigate the effects of different colon cancer microenvironment on HMSCbm. At the same time， HMSC-bm alone culture group and SW480 alone culture group were set up as control groups. The results found that with the increase of the proportion of colon cancer cells， the co-culture time was prolonged， the microscopic morphology of HMSC-bm cells was significantly changed， the proliferative capacity of HMSC-bm cells was enhanced， and the positive expression rate of CD34 was gradually increased; the positive rate of CD90 expression gradually cut back. This study indicates that the microenvironment of colon cancer may induce malignant transformation of HMSC-bm cells， and this malignant transformation is related to the proportion of cells and induction time.
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