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Di (2-ethylhexyl) Phthalate Inhibited Primordial Follicle by Inducing Autophagy in Mice Ovary
Zhang Yan, Mu Xinyi, Geng Yanqing, He Junlin, Liu Xueqing, Chen Xuemei, Ding Yubin, Wang Yingxiong, Gao Rufei*
Laboratory of the Reproductive Biology, School of Public Health and Management, Chongqing Medical University/Joint International Research Laboratory of Reproduction & Development, Chongqing 400016, China
Abstract: To investigate the effect of autophagy on Di (2-ethylhexyl) phthalate (DEHP)-inhibited primordial folliculogenesis in perinatal mice ovaries， the BALB/C mice were randomly divided into control group， DEHP group， DEHP and 3-MA combinative treatment group， and DEHP and Rapa combinative treatment group. The neonatal female mice were injected once a day from birth to 5 days postpartum (5 dpp)， and 16.5 days post coitum (dpc) fetal mice ovaries were cultured for 6 days. The results showed that the percent of primordial follicles decreased significantly and the percent of cyst-oocytes increased obviously in DEHP group， compared with control group. Furthermore， DEHP activated autophagy in the ovary， with increased autophagy-related gene expressions and recognizable autophagosomes， while inhibition of autophagy by 3-MA attenuated the adverse impact of DEHP on primordial folliculogenesis， moreover， induction of autophagy by Rapa strengthened the adverse impaction. This study demonstrated that perinatal exposure to DEHP induces autophagy to inhibit primordial folliculogenesis in mice ovary.
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