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Effects of Autophagy Inhibitor 3-MA on Mouse Embryo Implantation during the Peri-Implantation Period
Chen Mengyue, Gao Rufei, Li Na, Su Yan, Lu Siyu, Long Jing, He Junlin, Chen Xuemei, Liu Xueqing, Ding Yubing, Wang Yingxiong*
Laboratory of Reproductive Biology, School of Public Health and Management, Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Chongqing 400016, China
Abstract: Adult female Kunming mice were randomly divided into control group， 3-MA low dose group (15 mmol/L) and 3-MA high dose group (30 mmol/L). Animals were injected with autophagy inhibitor 3-MA until intraperitoneal， PBS for control. The expressions of Atg5 and LC3 protein after autophagy inhibitor 3-MA injection in endometrium were detected by Western blot. Morphological observation of control group and 3-MA
injection group was performed on the endometrial tissue of D4， D5 and D6. The expressions of CathepsinB， P62， progesterone receptor (PR) and estrogen receptor α (ERα) mRNA in the endometrium of D5 were detected by Realtime PCR. The expressions of Atg5， LC3 protein and CathepsinB， P62 mRNA of 3-MA injection group in the uterus of pregnant mice were significantly lower than that of the control group. After the injection of 3-MA autophagy inhibitor， the number of implantation sites (IS) in pregnant D6 was significantly lower than that in the control group. The expression of PR protein in 3-MA intervention group was significantly decreased and the expressions of PR and ERα mRNA were significantly decreased in the uterine endometrium inter-implantation sites (IIS) and implantation sites. With 3-MA inhibitor’s dose increased， PR and ER mRNA expression decreased much more. The results showed that autophagy inhibitor 3-MA might affect the endometrial receptivity of mouse during the periimplantation period， and its mechanism needs further study.
injection group was performed on the endometrial tissue of D4， D5 and D6. The expressions of CathepsinB， P62， progesterone receptor (PR) and estrogen receptor α (ERα) mRNA in the endometrium of D5 were detected by Realtime PCR. The expressions of Atg5， LC3 protein and CathepsinB， P62 mRNA of 3-MA injection group in the uterus of pregnant mice were significantly lower than that of the control group. After the injection of 3-MA autophagy inhibitor， the number of implantation sites (IS) in pregnant D6 was significantly lower than that in the control group. The expression of PR protein in 3-MA intervention group was significantly decreased and the expressions of PR and ERα mRNA were significantly decreased in the uterine endometrium inter-implantation sites (IIS) and implantation sites. With 3-MA inhibitor’s dose increased， PR and ER mRNA expression decreased much more. The results showed that autophagy inhibitor 3-MA might affect the endometrial receptivity of mouse during the periimplantation period， and its mechanism needs further study.
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