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shPLCε Can Inhibite Cell Migration and EMT of Prostate Cancer Cell via PPARβ/twist1
Fan Jiaxin1, Li Luo1, Niu Lingfang1, Fan Yanru1, Gao Yingying1, Wu Xiaohou2, Luo Chunli1*
1Key Laboratory of Diagnostics Medicine of Ministry of Education, Chongqing Medical University, Chongqing 400016, China; 2Department of Urinary Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Abstract: To investigate the effects of PLCε (phospholipase C epsilon) on EMT (epithelial-mesenchymal transition) and migration， we first build shPLCε cell lines by transfecting LV-shPLCε into prostate caner cell PC3 and DU145. q-PCR， Western blot， wound heling assay and Transwell experiment were used to investigate the effect of PLCε activity， EMT and migration induced by PLCε knockdown in PC3 and DU145. The results showed that shPLCε could significantly reduce both mRNA and protein levels of PLCε， PPARβ， and twist1. Wound heling assay and Transwell assay showed that the migration ability was remarkably decreased in the shPLCε group. At the same time， compared with the blank control group and shNC group， EMT was also decreased in shPLCε group (P<0.05). After adding the PPARβ agonist in shPLCε group， the expression of PPARβ and twist1 in both mRNA and protein levels， the migration ability and EMT were increased. The expression of PPARβ and twist1， migration ability and EMT were lower in shPLCε+GSK (PPARβ antagonists) group than those in shPLCε group.
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