Sec23a Inhibits the Stemness of Human Breast Cancer Stem-Like Cells

Abstract: The MDA-435-spheroid enrichment (MDA-435-SE) cells were isolated from human breast cancer cell line MDA-MB-435 through continuous spheroid-enrichment cell culture. Then the MDA-435-SEshSec23a cell line in which the Sec23a was knocked down and the negative control cell line MDA-435-SE-LV3NC was established through lentivirus infection. Cell Counting Kit-8 proliferation assay， 96-wells plate single cell cloning assay and nude mice subcutaneous tumor formation assay were conducted to figure out the effects of Sec23a stable knockdown on the stemness of MDA-435-SE cells. The results showed that Sec23a stable knockdown did not affect the cell proliferation rate of MDA-435-SE cells， but significantly enhanced the single cell cloning ability and tumorigenic ability of MDA-435-SE cells in vivo. This study illustrates that the stable knockdown of Sec23a enhances the stemness of human breast cancer stem-like cells.

CSTR: 32200.14.cjcb.2018.05.0003