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Isolation and Purification of Bone Marrow Hematopoietic Stem Cells in Mice and Electron Microscopy Observation
Zhang Xiaocui1, Gu Hao2, Zhao Benpeng1, Fu Rong1, Yu Zhuo2*
1Core Facility of Basic Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2Deparment of pathology and physiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
Abstract: The hematopoietic stem cells were isolated and purified from the mice bone marrow using immunomagnetic beads and flow cytometry， and observed by means of transmission electron microscopy. The results showed that 2×105 hematopoietic stem cells were collected from the 20 mice bone marrow， with a purity of more than 95%. They were single nuclear cells， similar to lymphocyte， suborbicular， about 5~7 μm in diameter， large nuclear/cytoplasmic ratios， uneven chromatin electron density. Other organelles were hard to observe， except for some mitochondria and vacuoles. It concluded that the purified mice bone marrow hematopoietic stem cells had high purity and high activity， which could be used as transmission electron microscope specimen and observed its ultrastructure characteristics clearly. This study attempts to find the character of mice bone marrow hematopoietic stem cells in morphology and provide basic data for future research， and to have certain application value in relationship with the structure and function.
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