The Effect of Long Non-Coding RNA 1500026H17Rik on Proliferation of Mesangial Cells under High Glucose Condition

Abstract: To explore the effect of long non-coding RNA 1500026H17Rik on proliferation of mesangial cells under high glucose condition， the level of 1500026H17Rik in tissues of diabetic nephropathy(DN) and mesangial cells under high and low glucose conditions was detected by quantitative Real-time PCR (qRTPCR); The full-length sequence of muse 1500026H17Rik was amplified. Then the amplified 1500026H17Rik was cloned into pcDNA3.1(+) vector and constructed pcDAN3.1(+)-1500026H17Rik over-expression plasmid. The 1500026H17Rik siRNA and pcDNA3.1(+)-1500026H17Rik over-expression plasmid were transfected into mesangial cells under high or low glucose condition. 5-ethyny1-2′-doexyuridine (EdU) assay was used to examine the cell proliferation ability. The results demonstrated that the level of 1500026H17Rik was significantly increased in kidney tissues of DN mice than that in control group， as well as， the level of 1500026H17Rik was increased in cells of high glucose group compared with that in low glucose group. Additionally， the over-expression plasmid pcDNA3.1(+)-1500026H17Rik was proved to be successful by enzyme digestion and DNA sequencing. And the proliferation ability was enhanced in pcDNA3.1(+)-1500026H17Rik group compared with that in low glucose mock group or control group. Moreover， the best efficiency of si1500026H17Rik was detected by qRT-PCR. And the proliferation ability was decreased in si1500026H17Rik group compared with that in high glucose mock group or control group. LncRNA-1500026H17Rik could regulate cell proliferation in mesangial cells. These results indicated that 1500026H17Rik significantly over-expresses in DN and may be associated with the mesangial cells proliferation in DN.

CSTR: 32200.14.cjcb.2018.04.0012