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LDHB Promotes the Growth of Glioma Cells via PDK1/Akt/GSK3β Pathway
Han Xiu, Chen Xi, Tang Yu, Cheng Jie, Liu Fei, Xu Jing, Peng Wanxin, Du Fengyi, Gong Aihua*
Department of Basic Medical Sciences, School of Medicine, Jiangsu University, Zhenjiang 212013, China
Abstract: The aim of the study is to investigate the effects of LDHB on the growth of human glioma cells in vivo. After transfecting the sh-LDHB or sh-EGFP plasmids into glioma cells， qRT-PCR and Western blot were used to detect the mRNA and protein levels of indicated genes. CCK-8 assay and colony formation assay were conducted to examine the proliferation of glioma cells ， flow cytometry was performed to invest the cycle and apoptosis. The results revealed that knockdown of LDHB reduced the proliferation of cells and resulted in sub-G1 DNA content， as well as decreased the proliferation related genes c-Myc and CylinD1 expression. Also， it inhibited the phosphorylation of PDK1， Akt and GSK3β. Taken together， LDHB played an important role in the growth of glioma cells via the PDK1/Akt/GSK3β signaling pathway.
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