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TGF-β1 Activated Hepatic Stellate Cells Induce Angiogenesis of Vascular Endothelial Cells
Jin Xin, Mao Xixian, Aimaiti Yasen, Chen Zixin, Li Dewei*
Department of Hepatobiliary Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Abstract: The aim of this study is to investigate the mechanism of transforming growth factor-beta 1 (TGF- β1) in the activation of hepatic stellate cells (HSCs)， and promoting effect of active HSCs on the angiogenesis. The mRNA levels of α-SMA， Smad2/3， VEGFA and TGF-β1-RI were determined by quantitative Real-time PCR; the protein levels of α-SMA， Smad2/3， VEGFA and TGF-β1-RI were determined by Western blot; the proangiogenic effect of activated hepatic stellate cells (HSCs) on human umbilical vein endothelial cells (HUVECs) was detected by Endothelial Cell Tube Formation Assay. TGF-β1 induced hepatic stellate cells (HSCs) can high express α-SMA and the downstream signal molecules of classical TGFβ1/TGF-β1-RI/Smad2/3 signaling pathways， while the HSCs get its proangiogenic potential. The study showed that TGF-β1 signaling activated HSCs through the classical Smad2/3 pathway， and the activated HSCs have the function of promoting the angiogenesis of vascular endothelial cells.
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