Home > Browse Issues > Vol.40 No.3
An Effective Method for Ammopiptanthus mongolicus Mitochondrial Genome Extraction
Yu Tingqiao, Cui Hongwei, Sun Lichun, Liu Shengli, Chen Yuzhen*, Lu Cunfu*
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
Abstract: Mitochondria is essential in the process of plant evolution. Xerophyte evergreen broadleaf shrub Ammopiptanthus mongolicus is a tertiary relic plant， which is mainly distributed in the desert region of northwest China. Analyses of the complete mitochondrial genome of A. mongolicus may provide valuable information for understanding the evolutionary adaptation of this xerophyte. However， it is difficult to isolate and purify the mitochondrial genome DNA of A. mongolicus using the conventional method. So， researches on A. mongolicus mitochondria function genome is restricted. In this study， we successfully obtained the high purity mitochondrial DNA by using the discontinuous sucrose density gradient centrifugation and DNase I digestion technology. The mitochondrial DNA purity and integrity met the requirements of building library for mitochondrial genome sequencing， supplying technological support for A. mongolicus functional mitochondria genome research.
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