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The Study on NF-κB Expression in the Endometreum of Mice Embryo Implantation at the Stage of Pre-Implantation
Liu Liyuan1, Jia Yongcun1, Yu Qiubo2*
1Clinical Laboratory Diagnostic Center, Ningxia People’ Hospital, the First affiliated Hospital of Northwest University for Nationalities, Yinchuan 750002, China; 2Molecular Medical Laboratory, Chongqing Medical University, Chongqing 400016, China
Abstract: NF-κB (nuclear factor of kappa B) is an important nuclear transcription involved in the inflammatory response， and embryo implantation is a physiological process is similar to the inflammation. This study examined the expression of NF-κB in endometrium of pregnancy mice (d1-d5) by Real-time PCR， Western blot and immunohistochemistry， and we detected the content of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in serum of pregnancy mice (d1-d5) by ELISA method. The study found that NF-κB was expressed on the first day of pregnancy and showed a gradual increased with the number of days of pregnancy. NF-κB was expressed in the endometrium of pseudopregnant mice and which was lower than that in the endometrium of pregnant mice at 5 d， and the expression of inflammatory cytokines IL-6 and TNF-α also increased with the number of the pregnancy days. The results of this study indicated that NF-κB may participate in the physiological process of embryo implantation through the process of inflammatory reaction.
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