Effects of JUP on Migration and Invasion Ability of Human Gastric Cancer Cell Line SGC-7901 and Its Mechanism

Abstract: The purpose of this study was to investigate the effects of junction plakoglobin (JUP) knockdown on the migration and invasion of human gastric cancer cell line SGC-7901 and its mechanism. The lentiviral vector sequence of knockdown JUP gene and its negative control virus were constructed by shRNA lentivirus-mediated method. The lentivirus and its negative control virus were transfected into human gastric cancer cell SGC-7901. After transfection for 3 days， the transfection efficiency of the cells was detected by microscope fluorescence， finally get well silenced JUP gene cell and the control. Positive transfected cell lines were screened with puromycin， the Real-time fluorescence quantitative PCR and Western blot were used to detect the JUP mRNA and protein level. The migration and invasion ability of human gastric cancer SGC-7901 cells were detected by cell wound healing and Transwell chamber test. Western blot was used to detect the effect of JUP knockdown on the level of β-catenin. The results showed that human gastric cancer SGC-7901 stable cell line knockdown by JUP gene was successfully constructed by shRNA-mediated lentivirus. Compared with control， knockdown JUP gene could promote cell migration and invasion (P<0.05)， the level of β-catenin was increased (P<0.05). The above results indicated that knockdown of JUP gene expression in human gastric cancer SGC-7901 cells might promote the migration and invasion of gastric cancer cell SGC-7901， which might be related to its upregulation β-catenin and activation of Wnt signaling pathway.

CSTR: 32200.14.cjcb.2018.03.0005