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BaP inhibits BMP2-induced osteogenic differentiation of Mesenchymal Stem Cells Line C3H10T1/2 through AhR
An Liqin, Shi Qiong, Zhou Yiqing, Liu Hongxia, Cheng Yu, Zhang Ruyi, Yan Shujuan, Weng Yaguang*
Key Laboratory of Clinical Laboratory Diagnostics of Ministry Education, Faculty of Laboratory Medicine, Chongqing Medicine University, Chongqing 400016, China
Abstract: The aim of this study was to investigate the effect of Benzoapyrene on bone morphogenetic protein 2 (BMP2)-induced osteogenic differentiation of mesenchymal stem cells line C3H10T1/2 and the regulatory mechanism involved. C3H10T1/2 cells were infected with Ad-BMP2/Ad-GFP and the expression of BMP2 and aryl hydrocarbon receptor (AhR) were detected by RT-PCR. The mRNA level of BMP2 significantly increased (P<0.001)， however， the the mRNA level of AhR did not alter significantly. Different concentration of BaP was to treat C3H10T1/2 for 7 days， detected with alkaline phosphatase (ALP) activity and ALP staining， and for 14 days， detected with Alizarin red S staining to observe the effect of BaP on BMP2-induced osteogenic differentiation. The results showed that BaP inhibited BMP2-induced ALP activity (P<0.001) and calcium deposition in a dose-dependent manner. It was also found that BaP significantly reduced the protein levels of p-Smad1/5/8 and Runx2 (P<0.01， P<0.001). However， when added AhR antagonists (CH223191)， we found that CH223191 could partly reverse the toxicologic effects of BaP on oesteogenic differentiation， at the mean while， it partly rescued the inhibition effects of BaP on BMP2/Smad signal pathway (P<0.05). We concluded that BaP can inhibit BMP2- induced osteogenic differentiation of C3H10T1/2 cells via AhR and it involved in BMP2/Smad signaling pathway.
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