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Cellular Biological Effects of REGγ Downregulation on HaCat Cells
Huang Tingmei, Zhao Juan*
School of Life Sciences, East China Normal University, Shanghai 200241, China
Abstract: To investigate the effects of REGγ downregulation on the cell proliferation， apoptosis and cell cycle of HaCat cells， we got the siN and siR HaCat cell lines by siRNA transfection. FACS， MTT and Western blot were used to detect the influnence of regulating REGγ expression. The results indicated that the downregulation of REGγ could elevate the expression of p53， a tumor inhibitor， and p21， a cyclin-dependent kinases inhibitors. Hence， the cell cycle and proliferation of HaCat cells were inhibited， inducing apoptosis subsequently.
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