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Effects of Exhaustive Exercise Induced Oxidative Stress on Glutathione Synthesis in Rat Erythrocytes and Its Mechanism
Wang Yueming1#, Yu Zhenhai1#, Xiong Yanlei2, An Xiaojing3, Wang Zhuoya1, Ji Piyou1, Wang Zhiqiang1, Zhou Yang1, Shi Pengzhi1, Zhao Jiantao1, Zhao Dongmei1*, Xiong Yanlian1*
1Department of Human Anatomy, College of Basic Medicine, Binzhou Medical University, Yantai 264000, China; 2Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100000, China; 3Yantai Stomatological Hospital, Yantai 264000, China
Abstract: The main purpose of the present study was to investigate the effects of exhaustive exercise induced oxidative stress on glutathione synthesis in red blood cells. Rats were divided into sedentary control (C) and exhaustive running (E) groups. The E group was divided further into four subgroups: exhaustive exercise immediate (E0)， exhaustive rest 1 h (E1)， exhaustive rest 12 h (E12) and exhaustive rest 24 h (E24). The antioxidant capacity of GSH， GSSG， GSH/GSSG and TFG of red blood cells collected from different group were evaluated. L-cysteine transport and ferric-reducing antioxidant power (FRAP) value were studied. An oxidation system in vitro was constructed to determined the levels of L-cysteine transport， TFG and FRAP， respectively. Compared with control group， the GSH content， TFG content and GSH/GSSG ratio decreased significantly， meanwhile GSSG content increased significantly in E0 group. After exhaustive exercise， the rate of L-cysteine influx rate in red blood cells of rats decreased significantly， and the level of FRAP decreased significantly. Under in vitro oxidative conditions， the L-cysteine influx rate and FRAP levels significantly decreased in control group. We present evidence of exhaustive exercise induced oxidative stress in erythrocytes of rats， resulting in a decrease in L-cysteine transport rate and a decrease in the synthesis efficiency of GSH， a major antioxidant in red blood cells. These changes have led to a further decrease in the antioxidant capacity of erythrocytes and thus become a potential causative factor in exhaustive exercise injury.
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