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The Study of Proliferation of Cervical Carcinoma Promoted by HOXA7 in Vitro
Chen Jun1,2,3, Li Yuehui1,2, Liu Zhaoyang1,2, Li Wenling1,2, Yang Xiaohui4,5*, Li Guancheng1,2*
1The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health and the Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Xiangya Hospital, Central South University, Changsha 410008, China; 2Cancer Research Institute, Central South University, Changsha 410078, China; 3Hunan University of Environment and Biology, Hengyang 421005, China; 4Department of Public Health, Central South University, Changsha 410078, China; 5The Xiangya Third Hospital, Central South University, Changsha 410013, China
Abstract: This study investigated the effect of Siha Caski in vitro proliferation of cervical cancer cells after knockdown HOXA7 gene. Then HOXA7 gene si-RNA was stably transfected into Siha and Caski cells， respectively. The effects of RNA interference were identified by RT-PCR and Western blot. The growth rate of Siha and Caski cells was detected by MTT [3-(4，5-Dimethylthiazol-2-yl)-2，5-diphenyltetrazolium bromide] assay and cell do time test after knockdown of HOXA7 gene. The clonal formation ability of Siha and Caski cells was detected by plate cloning assay， and the cell cycle of Siha and Caski cells was detected by flow cytometry after knockdown HOXA7 gene. The survial rate of cell inoculation was detected by a flatcloneformation test， flow cytometry was used to detect cell cycle. The results showed that the expression of HOXA7 was down regulated in Siha and Caski cells after the knockdown of HOXA7 gene. MTT results showed that the growth rate of Siha/si-HOXA7 and Caski/ si-HOXA7 was decreased significantly in the experimental group. It was found that the average doubling times of Siha/NC， Caski/NC， Siha/si-HOXA7 and Caski/si-HOXA7 were 5.652±0.352， 4.650±0.340， 7.342±0.331 and 6.987±0.330 h， respectively. The clone formation rates of Siha/NC， Caski/NC， Siha/si-HOXA7 and Caski/si- HOXA7 were 35.400%±1.429%， 31.700%±1.943%， 24.200%±1.098% and 21.200%±1.838%， respectively. The cells difference between the control group and the experimental group was statistically significant (P<0.01). The cell cycle of the experimental group also changed: phase G1 cells increased and phase S cells decreased. This showed that HOXA7 gene could promote the proliferation of cervical cancer cells Caski and Siha， which laid a solid foundation for further exploring the function of HOXA7 gene and studying the pathogenesis of cervical cancer.
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