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The Role of p11 in Nerve Cells Autophagy and Its Underlying Mechanism
Feng Laipeng1,2,3, Guo Jiqiang1,2,3, Yang Tingting1,2,3, Shen Ying1,2,3, Wang Hui1,2,3*
1Research Central for Immunology, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453000, China; 2Henan Province Molecular Diagnostics and Medical Laboratory Technology Collaborative Innovation Center, Xinxiang 453000, China; 3Henan Province Key Laboratory of Immunization and Targeted Drugs, Xinxiang 453000, China
Abstract: This study is to explore the effect of p11 (also known as S100A10) on the autophagy of both neuroblastoma cell line (SH-SY5Y) and mouse hippocampal cells and its mechanism. Both the plasmid of over-expression of p11 (pcDNA3.0-p11) and the plasmid of gene knockdown of p11 (Si-p11) were transfected into SH-SY5Y. Then after 24 h， total protein and RNA of SH-SY5Y were extracted which were measured the expression of autophagy-related genes mRNAs and proteins. Then， C57BL/6J mice were injected imipramine into abdominal cavity to raise the expression level of p11 in hippocampus. The protein and RNA of hippocampus in injected C57BL/6J mice were extracted to detect the expression of autophagy-related genes. The results of the cell-experiments showed that the overexpression of p11 had a down-regulation effect on autophagy of nerve cells， and gene knockdown of p11 had an up-regulation effect on autophagy of nerve cells. The autophagy of hippocampal cells in mice without p11 was up-regulated， and the autophagy of hippocampal cells in mice with p11 overexpression was down-regulated. Therefore， we believe that p11 may play a down-regulated role in the autophagy of nerve cells.
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