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Protein Expression of Monoclonal Epithelia Stem Cells Derived from Pancreatic Duct
Wang Ruiyang, Li Yanwei, Lan Ruixia, Xu Yingmei, Xiao Mei*, An Lilong*
Department of Animal Science and Medicine, Guangdong Ocean University, Zhanjiang 524088, China
Abstract: In this study， expression characteristics， karyotype and tumorigenicity of a monoclonal epithelia stem cell line derived from pancreatic duct of an adult rat were researched. Monoclonal epithelia stem cells derived from pancreatic duct of an adult rat were proliferated to a layer in serum supplemented RPMI-1640 medium. Expression characteristics of stem cells were determined using flow cytometry and immunofluorescence reaction. Chromosome sets of stem cells were examed using conventional method， and karyotype was analysed with Adobe Photoshop CS6 software. Tumorigenicity was observed by transplanting stem cells into nude mice. The results showed that monoclonal epithelia stem cells derived from pancreatic duct of a adult rat were positive for the CK19， NeuroD2， Oct4， PCNA and Nanog， and were negative for the Pdx1， Pax4， Pax6， MafA， Ptf1a， Ngn3， nestin， Sox2， CD34， CD45， insulin， glucagon， ghrelin， somatostatin and secretin protein expression. Stem cells were normal diploid (2n=42). And these stem cells had no oncogenicity. Here a monoclonal epithelia stem cell line derived from pancreatic duct of an adult rat that co-expressed the CK19， NeuroD2， Oct4， PCNA and Nanog protein was determined.
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