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Knockdown of Ski Gene Expression Influence the Proliferation and Migration Abilities of Osteosarcoma U-2OS Cells
Kou Jiangli1,2#, Zhao Xin1,2#, Wang Xingwen1,2, Wang Jing2, Wang Shuanke1, Zhang Haihong1*
1Department of Orthopedics, Second Clinical Medical College of Lanzhou University, Lanzhou 730030, China; 2Key Laboratory of Orthopedics of Gansu Province, Lanzhou 730030, China
Abstract: The effect of Ski gene expression on the proliferation and migration of human osteosarcoma U-2OS cells in vitro was studied in this paper. The Ski gene-knockdown U-2OS cell line was successfully constructed. The protein level of Ski in U-2OS cells was significantly decreased after transfection with Ski-siRNA (P<0.05). The proliferation vitalities of U-2OS cells at 12， 24 and 48 h after Ski gene-knockdown were decreased than those in the blank and negative groups (P<0.05). At the same time， the cells percentage of G1 phase was increased after Ski gene-knockdown， while S and G2 phases were significantly decreased (P<0.05). In wound healing assay， the wound healing rate of Ski-siRNA group was significantly lower than that of the negative control siRNA group in 12， 24， 48 h (P<0.05). Furthermore， the protein levels of PCNA， cyclin D1， MMP-2 and MMP-9 were also decreased in Ski-siRNA group (P<0.05). These results indicated that knockdown of Ski gene by siRNA significantly decreased the proliferation and migration abilities of human osteosarcoma U-2OS cells. They indicated that Ski gene might become a new potential targeting gene in the therapy for human osteosarcoma disease.
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